Publications
37. Uddin, N., Dinar, M.A.M., Schrass, L.E., Pack, D.W., DeRouchey, J.E.*. Impact of Acetylation, Succinylation, and pH on DNA packaging in PEI-Based Polyplexes, 2025, Biomacromolecules, 26, 1, 178-189. (link)
36. Ahmed, A. J., Gallegos, Z. A., Dinar, M.A.M., Sullivan, P.G., DeRouchey, J.E., Patel, S.P., Rabchevsky, A.G., Dziubla, T.D.* "Physicochemical Characterization of Hyaluronic Acid-Methylcellulose Semi-gels for Mitochondria Transplantation, 2024, Journal of Biomedical Materials Research: Part B - Applied Biomaterials, in press. (link)
35. Oikeh, E., Ziebarth, J., Kirchhoff, D., Aronova, A., Dziubla, T.D., Wang, Y. DeRouchey, J.E.*. DNA Packaging and Polycation Length Determine DNA Susceptibility to Free Radical Damage in Condensed DNA, 2024, J. Phys. Chem. B, 128, 14, 3329–3339. (link)
34. Mitchell, R.J., Kriger, S.M., Fenton, A.D., Sun, Y., Havrylyuk, D., Pandeya, A., Smith, T., Wei, Y., Unrine, J.M., DeRouchey, J.E., Heidary, D.K.*, Glazer, E.C.*, A Monoadduct Generating Ruthenium Complex Induces Ribosome Biogenesis Stress and is a Molecular Mimic of Phenanthriplatin, 2023, RSC Chem. Biol., 2023,4, 344-353. (link)
33. Shadman, H., Gallops, C., Ziebarth, J., DeRouchey, J.E., Wang, Y.*, Exploring Structures and Dynamics of Protamine Molecules through Molecular Dynamics Simulations, 2022, ACS Omega, 7, 46, 42083–42095. (link)
32. Patel, S.P., Michael, F.M., Arif, Khan M., Duggan, B., Wyse, S., Darby, D.R., Chaudhuri, K., Pham, J.T., Gollihue, J., DeRouchey, J.E., Sullivan, P.G., Dziubla, T.D., Rabchevsky, A.G.* Erodible thermogelling hydrogels for localized mitochondrial transplantation to the spinal cord, 2022, Mitochondrion, 2022, 64(1), 145-155. (link)
31. Saintilnord, W.N., Tenlep, S.N.Y., Preston, J.D., Duregon, E., DeRouchey, J.E., Unrine, J.M., de Cabo, R., Pearson, K.J.*, Fondufe-Mittendorf, Y.N.* Chronic Exposure to Cadmium Induces Differential Methylation in Mice Spermatozoa, Toxicological Sciences, 2021, 180(2), 262-276. (link)
30. Uddin, N., Warriner, L.W., Pack D.W.*, DeRouchey, J.E.* Enhanced Gene Delivery and CRISPR/Cas9 Homology-Directed Repair in Serum by Minimally Succinylated Polyethylenimine. Molecular Pharmaceutics (2021), 18(9), 3452–3463. (link)
29. Lichtenberg S.S., Nuti, K., DeRouchey, J.E., Tsyusko, O.V., Unrine, J.M.* Efficacy of chitosan/double-stranded RNA polyplex nanoparticles for gene silencing under variable environmental conditions. Environ. Sci.: Nano, 2020, 7(5), 1582-1592 (link)
28. Powell, C.D., Kirchoff, D.C., DeRouchey, J.E., Moseley, H.N.B.* Entropy based analysis of vertebrate sperm protamines sequences: evidence of potential dityrosine and cysteine-tyrosine cross-linking in sperm protamines. BMC Genomics (2020), 21, 277. (link)
27. Fu, X., Song, Y., Masud, A. Nuti, K., DeRouchey, J.E., Richards, C.I.* High-throughput fluorescence correlation spectroscopy enables analysis of surface components of cell-derived vesicles. Anal Bioanal Chem 2020, 412, 2589–2597. (link)
26. Warriner L. W.; Duke, III, J. R.; Pack, D. W.*; DeRouchey, J. E.*, Succinylated Polyethylenimine Derivatives Greatly Enhance Polyplex Serum Stability and Gene Delivery In Vitro. Biomacromolecules 2018, 19 (11), 4348-4357. (link)
25. Hansing, J.; Duke, III, J. R.; Fryman, E. B.; DeRouchey, J. E.*; Netz, R. R.*, Particle Diffusion in Polymeric Hydrogels with Mixed Attractive and Repulsive Interactions. Nano Letters 2018, 18 (8), 5248-5256. (link)
24. An, M.; Tonga, G. Y.; Parkin, S. R.; Rotello, V. M.; DeRouchey, J. E.*, Tuning DNA Condensation with Zwitterionic Polyamidoamine (zPAMAM) Dendrimers. Macromolecules 2017, 50 (20), 8202-8211. (link)
23. Hutchison, J.M.; Rau, D.C.; DeRouchey J.E.* Role of Disulfide Bonds on DNA Packaging Forces in Bull Sperm Chromatin. Biophys J. 2017, 113 (9), 1925-1933. (link)
22. Stocke, N. A.; Zhang, X.; Hilt, J. Z.; DeRouchey, J. E.*, Transport in PEG-Based Hydrogels: Role of Water Content at Synthesis and Crosslinker Molecular Weight. Macromolecular Chemistry and Physics 2017, 218 (3), 1600340. (link)
21.Hansing, J.; Ciemer, C.; Kim, W. K.; Zhang, X.; DeRouchey, J. E.; Netz, R. R., Nanoparticle Filtering in Charged Hydrogels: Effects of Particle Size, Charge Asymmetry and Salt Concentration. European Physics Journal E Soft Matter 2016, 39 (5), 53. (link)
20. Zhang, X.; Hansing, J.; Netz, Roland R.; DeRouchey, J. E.*, Particle Transport through Hydrogels Is Charge Asymmetric. Biophysical Journal 2015, 108 (3), 530-539. (link)
19. Moonschi, F. H.; Effinger, A. K.; Zhang, X.; Martin, W. E.; Fox, A. M.; Heidary, D. K.; DeRouchey, J. E.; Richards, C. I., Cell-Derived Vesicles for Single-Molecule Imaging of Membrane Proteins. Angewandte Chemie 2015, 127 (2), 491-494. (link)
18. An, M.; Parkin, S. R.; DeRouchey, J. E.*, Intermolecular Forces Between Low Generation PAMAM Dendrimer Condensed DNA Helices: Role of Cation Architecture. Soft Matter 2014, 10 (4), 590-599. (link)
17.An, M.; Hutchison, J. M.; Parkin, S. R.; DeRouchey, J. E.*, Role of pH on the Compaction Energies and Phase Behavior of Low Generation PAMAM-DNA Complexes. Macromolecules 2014, 47 (24), 8768–8776. (link)
16. DeRouchey, J.; Hoover, B.; Rau, D. C., A Comparison of DNA compaction by Arginine and Lysine Peptides: A Physical Basis for Arginine Rich Protamines. Biochemistry 2013, 52 (17), 3000-3009. (link)
15. DeRouchey, J. E.*; Rau, D. C., Salt Effects on Condensed Protamine-DNA Assemblies: Anion Binding and Weakening of Attraction. Journal of Physical Chemistry B 2011, 115 (41), 11888-11894. (link)
14. DeRouchey, J. E.*; Rau, D. C., Role of Amino Acid Insertions on Intermolecular Forces Between Arginine Peptide Condensed DNA Helices: Implications for Protamine-DNA Packaging in Sperm. Journal of Biological Chemistry 2011, 286 (49), 41985-41992. (link)
Prior Publications
13. Hoenig, D.; DeRouchey, J.*; Jungmann, R.; Koch, C.; Plank, C.; Rädler, J. O., Biophysical Characterization of Copolymer-Protected Gene Vectors. Biomacromolecules 2010, 11 (7), 1802-1809. (link)
12. DeRouchey, J.*; Parsegian, V. A.; Rau, D. C., Cation Charge Dependence of the Forces Driving DNA Assembly. Biophysical Journal 2010, 99 (8), 2608-2615. (link)
11. DeRouchey, J.*; Schmidt, C.; Walker, G. F.; Koch, C.; Plank, C.; Wagner, E.; Rädler, J. O., Monomolecular Assembly of siRNA and Poly(ethylene glycol)-Peptide Copolymers. Biomacromolecules2008, 9 (2), 724-732. (link)
10. Pelisek, J.; Gaedtke, L.; DeRouchey, J.; Walker, G. F.; Niko, S.; Wagner, E., Optimized Lipopolyplex Formulations for Gene Transfer to Human Colon Carcinoma Cells Under In Vitro Conditions. Journal of Gene Medicine 2006, 8 (2), 186-197. (link)
09. DeRouchey, J.*; Walker, G. F.; Wagner, E.; Rädler, J. O., Decorated Rods: A "Bottom-up" Self-Assembly of Monomolecular DNA Complexes. Journal of Physical Chemistry B 2006, 110 (10), 4548-4554. (link)
08. DeRouchey, J.*; Netz, R. R.; Rädler, J. O., Structural investigations of DNA-Polycation Complexes. European Physical Journal E 2005, 16 (1), 17-28. (link)
07. DeRouchey, J.; Thurn-Albrecht, T.; Russell, T. P.; Kolb, R., Block Copolymer Domain Reorientation in an Electric Field: An in-situ Small-Angle X-ray Scattering Study. Macromolecules 2004, 37 (7), 2538-2543. (link)
06. Thurn-Albrecht, T.; DeRouchey, J.; Russell, T. P.; Kolb, R., Pathways Toward Electric Field Induced Alignment of Block Copolymers. Macromolecules 2002, 35 (21), 8106-8110. (link)
05. Xu, T.; Kim, H. C.; DeRouchey, J.; Seney, C.; Levesque, C.; Martin, P.; Stafford, C. M.; Russell, T. P., The Influence of Molecular Weight on Nanoporous Polymer Films. Polymer 2001, 42 (21), 9091-9095. (link)
04. Thurn-Albrecht, T.; Steiner, R.; DeRouchey, J.; Stafford, C. M.; Huang, E.; Bal, M.; Tuominen, M.; Hawker, C. J.; Russell, T., Nanoscopic Templates From Oriented Block Copolymer Films. Advanced Materials 2000, 12 (11), 787-791. (link)
03. Thurn-Albrecht, T.; DeRouchey, J.; Russell, T. P.; Jaeger, H. M., Overcoming Interfacial Interactions with Electric Fields. Macromolecules 2000, 33 (9), 3250-3253. (link)
02. Boal, A. K.; Ilhan, F.; DeRouchey, J. E.; Thurn-Albrecht, T.; Russell, T. P.; Rotello, V. M., Self-Assembly of Nanoparticles into Structured Spherical and Network Aggregates. Nature 2000, 404 (6779), 746-748. (link)
01. Mansky, P.; DeRouchey, J.; Russell, T. P.; Mays, J.; Pitsikalis, M.; Morkved, T.; Jaeger, H., Large-Area Domain Alignment in Block Copolymer Thin Films Using Electric Fields. Macromolecules1998, 31 (13), 4399-4401. (link)
Book Contributor
01. Podgornik, R.; Harries, D.; DeRouchey, J.; Parsegian, V. A.; Strey, H. H., “Interactions in Macromolecular Complex Used as Nonviral Vectors for Gene Delivery.’ In Gene and Cell Therapy: Therapeutic Mechanisms and Strategies; Third Edition. Templeton, N.S. ed; Marcel Dekker: NewYork, 2009; pp 443-484.
36. Ahmed, A. J., Gallegos, Z. A., Dinar, M.A.M., Sullivan, P.G., DeRouchey, J.E., Patel, S.P., Rabchevsky, A.G., Dziubla, T.D.* "Physicochemical Characterization of Hyaluronic Acid-Methylcellulose Semi-gels for Mitochondria Transplantation, 2024, Journal of Biomedical Materials Research: Part B - Applied Biomaterials, in press. (link)
35. Oikeh, E., Ziebarth, J., Kirchhoff, D., Aronova, A., Dziubla, T.D., Wang, Y. DeRouchey, J.E.*. DNA Packaging and Polycation Length Determine DNA Susceptibility to Free Radical Damage in Condensed DNA, 2024, J. Phys. Chem. B, 128, 14, 3329–3339. (link)
34. Mitchell, R.J., Kriger, S.M., Fenton, A.D., Sun, Y., Havrylyuk, D., Pandeya, A., Smith, T., Wei, Y., Unrine, J.M., DeRouchey, J.E., Heidary, D.K.*, Glazer, E.C.*, A Monoadduct Generating Ruthenium Complex Induces Ribosome Biogenesis Stress and is a Molecular Mimic of Phenanthriplatin, 2023, RSC Chem. Biol., 2023,4, 344-353. (link)
33. Shadman, H., Gallops, C., Ziebarth, J., DeRouchey, J.E., Wang, Y.*, Exploring Structures and Dynamics of Protamine Molecules through Molecular Dynamics Simulations, 2022, ACS Omega, 7, 46, 42083–42095. (link)
32. Patel, S.P., Michael, F.M., Arif, Khan M., Duggan, B., Wyse, S., Darby, D.R., Chaudhuri, K., Pham, J.T., Gollihue, J., DeRouchey, J.E., Sullivan, P.G., Dziubla, T.D., Rabchevsky, A.G.* Erodible thermogelling hydrogels for localized mitochondrial transplantation to the spinal cord, 2022, Mitochondrion, 2022, 64(1), 145-155. (link)
31. Saintilnord, W.N., Tenlep, S.N.Y., Preston, J.D., Duregon, E., DeRouchey, J.E., Unrine, J.M., de Cabo, R., Pearson, K.J.*, Fondufe-Mittendorf, Y.N.* Chronic Exposure to Cadmium Induces Differential Methylation in Mice Spermatozoa, Toxicological Sciences, 2021, 180(2), 262-276. (link)
30. Uddin, N., Warriner, L.W., Pack D.W.*, DeRouchey, J.E.* Enhanced Gene Delivery and CRISPR/Cas9 Homology-Directed Repair in Serum by Minimally Succinylated Polyethylenimine. Molecular Pharmaceutics (2021), 18(9), 3452–3463. (link)
29. Lichtenberg S.S., Nuti, K., DeRouchey, J.E., Tsyusko, O.V., Unrine, J.M.* Efficacy of chitosan/double-stranded RNA polyplex nanoparticles for gene silencing under variable environmental conditions. Environ. Sci.: Nano, 2020, 7(5), 1582-1592 (link)
28. Powell, C.D., Kirchoff, D.C., DeRouchey, J.E., Moseley, H.N.B.* Entropy based analysis of vertebrate sperm protamines sequences: evidence of potential dityrosine and cysteine-tyrosine cross-linking in sperm protamines. BMC Genomics (2020), 21, 277. (link)
27. Fu, X., Song, Y., Masud, A. Nuti, K., DeRouchey, J.E., Richards, C.I.* High-throughput fluorescence correlation spectroscopy enables analysis of surface components of cell-derived vesicles. Anal Bioanal Chem 2020, 412, 2589–2597. (link)
26. Warriner L. W.; Duke, III, J. R.; Pack, D. W.*; DeRouchey, J. E.*, Succinylated Polyethylenimine Derivatives Greatly Enhance Polyplex Serum Stability and Gene Delivery In Vitro. Biomacromolecules 2018, 19 (11), 4348-4357. (link)
25. Hansing, J.; Duke, III, J. R.; Fryman, E. B.; DeRouchey, J. E.*; Netz, R. R.*, Particle Diffusion in Polymeric Hydrogels with Mixed Attractive and Repulsive Interactions. Nano Letters 2018, 18 (8), 5248-5256. (link)
24. An, M.; Tonga, G. Y.; Parkin, S. R.; Rotello, V. M.; DeRouchey, J. E.*, Tuning DNA Condensation with Zwitterionic Polyamidoamine (zPAMAM) Dendrimers. Macromolecules 2017, 50 (20), 8202-8211. (link)
23. Hutchison, J.M.; Rau, D.C.; DeRouchey J.E.* Role of Disulfide Bonds on DNA Packaging Forces in Bull Sperm Chromatin. Biophys J. 2017, 113 (9), 1925-1933. (link)
22. Stocke, N. A.; Zhang, X.; Hilt, J. Z.; DeRouchey, J. E.*, Transport in PEG-Based Hydrogels: Role of Water Content at Synthesis and Crosslinker Molecular Weight. Macromolecular Chemistry and Physics 2017, 218 (3), 1600340. (link)
21.Hansing, J.; Ciemer, C.; Kim, W. K.; Zhang, X.; DeRouchey, J. E.; Netz, R. R., Nanoparticle Filtering in Charged Hydrogels: Effects of Particle Size, Charge Asymmetry and Salt Concentration. European Physics Journal E Soft Matter 2016, 39 (5), 53. (link)
20. Zhang, X.; Hansing, J.; Netz, Roland R.; DeRouchey, J. E.*, Particle Transport through Hydrogels Is Charge Asymmetric. Biophysical Journal 2015, 108 (3), 530-539. (link)
19. Moonschi, F. H.; Effinger, A. K.; Zhang, X.; Martin, W. E.; Fox, A. M.; Heidary, D. K.; DeRouchey, J. E.; Richards, C. I., Cell-Derived Vesicles for Single-Molecule Imaging of Membrane Proteins. Angewandte Chemie 2015, 127 (2), 491-494. (link)
18. An, M.; Parkin, S. R.; DeRouchey, J. E.*, Intermolecular Forces Between Low Generation PAMAM Dendrimer Condensed DNA Helices: Role of Cation Architecture. Soft Matter 2014, 10 (4), 590-599. (link)
17.An, M.; Hutchison, J. M.; Parkin, S. R.; DeRouchey, J. E.*, Role of pH on the Compaction Energies and Phase Behavior of Low Generation PAMAM-DNA Complexes. Macromolecules 2014, 47 (24), 8768–8776. (link)
16. DeRouchey, J.; Hoover, B.; Rau, D. C., A Comparison of DNA compaction by Arginine and Lysine Peptides: A Physical Basis for Arginine Rich Protamines. Biochemistry 2013, 52 (17), 3000-3009. (link)
15. DeRouchey, J. E.*; Rau, D. C., Salt Effects on Condensed Protamine-DNA Assemblies: Anion Binding and Weakening of Attraction. Journal of Physical Chemistry B 2011, 115 (41), 11888-11894. (link)
14. DeRouchey, J. E.*; Rau, D. C., Role of Amino Acid Insertions on Intermolecular Forces Between Arginine Peptide Condensed DNA Helices: Implications for Protamine-DNA Packaging in Sperm. Journal of Biological Chemistry 2011, 286 (49), 41985-41992. (link)
Prior Publications
13. Hoenig, D.; DeRouchey, J.*; Jungmann, R.; Koch, C.; Plank, C.; Rädler, J. O., Biophysical Characterization of Copolymer-Protected Gene Vectors. Biomacromolecules 2010, 11 (7), 1802-1809. (link)
12. DeRouchey, J.*; Parsegian, V. A.; Rau, D. C., Cation Charge Dependence of the Forces Driving DNA Assembly. Biophysical Journal 2010, 99 (8), 2608-2615. (link)
11. DeRouchey, J.*; Schmidt, C.; Walker, G. F.; Koch, C.; Plank, C.; Wagner, E.; Rädler, J. O., Monomolecular Assembly of siRNA and Poly(ethylene glycol)-Peptide Copolymers. Biomacromolecules2008, 9 (2), 724-732. (link)
10. Pelisek, J.; Gaedtke, L.; DeRouchey, J.; Walker, G. F.; Niko, S.; Wagner, E., Optimized Lipopolyplex Formulations for Gene Transfer to Human Colon Carcinoma Cells Under In Vitro Conditions. Journal of Gene Medicine 2006, 8 (2), 186-197. (link)
09. DeRouchey, J.*; Walker, G. F.; Wagner, E.; Rädler, J. O., Decorated Rods: A "Bottom-up" Self-Assembly of Monomolecular DNA Complexes. Journal of Physical Chemistry B 2006, 110 (10), 4548-4554. (link)
08. DeRouchey, J.*; Netz, R. R.; Rädler, J. O., Structural investigations of DNA-Polycation Complexes. European Physical Journal E 2005, 16 (1), 17-28. (link)
07. DeRouchey, J.; Thurn-Albrecht, T.; Russell, T. P.; Kolb, R., Block Copolymer Domain Reorientation in an Electric Field: An in-situ Small-Angle X-ray Scattering Study. Macromolecules 2004, 37 (7), 2538-2543. (link)
06. Thurn-Albrecht, T.; DeRouchey, J.; Russell, T. P.; Kolb, R., Pathways Toward Electric Field Induced Alignment of Block Copolymers. Macromolecules 2002, 35 (21), 8106-8110. (link)
05. Xu, T.; Kim, H. C.; DeRouchey, J.; Seney, C.; Levesque, C.; Martin, P.; Stafford, C. M.; Russell, T. P., The Influence of Molecular Weight on Nanoporous Polymer Films. Polymer 2001, 42 (21), 9091-9095. (link)
04. Thurn-Albrecht, T.; Steiner, R.; DeRouchey, J.; Stafford, C. M.; Huang, E.; Bal, M.; Tuominen, M.; Hawker, C. J.; Russell, T., Nanoscopic Templates From Oriented Block Copolymer Films. Advanced Materials 2000, 12 (11), 787-791. (link)
03. Thurn-Albrecht, T.; DeRouchey, J.; Russell, T. P.; Jaeger, H. M., Overcoming Interfacial Interactions with Electric Fields. Macromolecules 2000, 33 (9), 3250-3253. (link)
02. Boal, A. K.; Ilhan, F.; DeRouchey, J. E.; Thurn-Albrecht, T.; Russell, T. P.; Rotello, V. M., Self-Assembly of Nanoparticles into Structured Spherical and Network Aggregates. Nature 2000, 404 (6779), 746-748. (link)
01. Mansky, P.; DeRouchey, J.; Russell, T. P.; Mays, J.; Pitsikalis, M.; Morkved, T.; Jaeger, H., Large-Area Domain Alignment in Block Copolymer Thin Films Using Electric Fields. Macromolecules1998, 31 (13), 4399-4401. (link)
Book Contributor
01. Podgornik, R.; Harries, D.; DeRouchey, J.; Parsegian, V. A.; Strey, H. H., “Interactions in Macromolecular Complex Used as Nonviral Vectors for Gene Delivery.’ In Gene and Cell Therapy: Therapeutic Mechanisms and Strategies; Third Edition. Templeton, N.S. ed; Marcel Dekker: NewYork, 2009; pp 443-484.